Methodological Advances & Chemometrics in Membrane Biophysics
We develop and refine physicochemical techniques—including FTIR, UV-Vis, and refractometry—to track molecular-level phenomena at the membrane-water interface. By combining vibrational and electronic spectroscopy with chemometric analysis, we reconstruct fine structural features and resolve subtle changes in orientation and hydration of the particular functional groups at the membrane surface.
Related Research Papers
Pem, Barbara; Pišonić, Marina; Momčilov, Marina; Crnolatac, Ivo; Brkljača, Zlatko; Vazdar, Mario; Bakarić, Danijela
Protonation of palmitic acid embedded in DPPC lipid bilayers obscures detection of ripple phase by FTIR spectroscopy // Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 322 (2024), 124773, 11. doi: 10.1016/j.saa.2024.124773
Maleš, Petra ; Brkljača, Zlatko ; Domazet Jurašin, Darija ; Bakarić, Danijela
New spirit of an old technique: Characterization of lipid phase transitions via UV/Vis spectroscopy // Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 272 (2022), 121013, 7. doi: 10.1016/j.saa.2022.121013
Maleš, Petra ; Brkljača, Zlatko ; Crnolatac, Ivo ; Bakarić, Danijela
Application of MCR-ALS with EFA on FT-IR Spectra of Lipid Bilayers in the Assessment of Phase Transition Temperatures: Potential for Discernment of Coupled Events // Colloids and surfaces. B, Biointerfaces, 201 (2021), 111645, 8. doi: 10.1016/j.colsurfb.2021.111645